The centrifugal force in the shake flask can be calculated by the following formula:

FC = rpm2 × amplitude

There is a linear relationship between centrifugal force and amplitude: if Ø25mm to Ø50mm (same rotation speed) is used, the centrifugal force is doubled

There is a square relationship between centrifugal force and speed:

If the speed is increased to 2 times (the same amplitude), the centrifugal force is increased by 4 times. If the speed is increased to 3 times, the centrifugal force is increased by 9 times!

If Ø25mm is used, the culture is performed at a given speed. If you want to achieve the same centrifugal force with Ø50mm, the speed should be calculated as 1/2 square root, so you should use 70% speed to achieve the same culture conditions.

Please note that the above is a theoretical calculation of centrifugal force. There will be other influencing factors in real applications. This calculation method can get approximate values for operation reference.

Inexperienced buyers of orbital shakers often neglect to take into account orbit diameter when selecting an instrument, not realizing how important it is. The orbit diameter of an instrument determines what applications and vessels it is more suitable for.

Ø  As a rule of thumb, consider the following when determining which orbit is right for you:

1、3 mm (about 1/8"): Best for microplates, microcentrifuge tubes, and other very small vessels.

2、15 mm - 25 mm (about 1/2" - 1"): Best for cell culture dishes, and flasks and beakers up to about 2 liters.

3、30+ mm: Best for large vessels above 2 liters or especially wide vessels.

Ø  What if your vessel is on the cusp? For instance, say you want to shake 50 ml centrifuge tubes in a vertical position? The good thing about orbital shakers is that many vessels aren't overly sensitive to the orbit diameter. In the case of 50 ml tubes, you could use a shaker with a 3 mm diameter or an ~20 mm diameter.

Ø  That said, for applications that aren't simple mixing, such as cell / bacterial culture, things get a little more complicated and involve things like aeration. Let's say you have a protocol that says you should shake at 25 mm but your orbital shaker has a 15 mm diameter. What can you do?

4、You can usually compensate for a somewhat different orbit diameter (say, +/- 50% of what a protocol calls for) by changing the speed. If your orbit diameter is smaller than what your protocol calls for, simply turn up the RPM a bit to compensate. Conversely, if the orbit diameter was too large you would decrease the speed somewhat.

Ø  So don't worry about orbit diameter - it doesn't need to be perfect - but do be aware of orbit diameter, as it's more important than a lot of people realize.

Zhichu offers shakers with amplitudes of Ø1.5mm, Ø3mm, Ø26mm, and Ø50mm.

Light is also one of the important conditions in tissue culture, mainly in terms of light intensity, light quality, and light time:

1.Light intensity

Light intensity has an important effect on the proliferation of cells and differentiation of organs. From the current research situation, light intensity has a significant effect on the initial division of plants and cells. Generally speaking, the light intensity is strong and the seedlings grow stout, while the light intensity is weak and the seedlings are easy to grow.

Zhichu provides shakers with adjustable light intensity of 1% -100% step and 1% as well as a variety of light sources.

2.Light quality

Light quality has a significant effect on callus induction, proliferation of cultured tissues, and differentiation of organs. For example, lily bulbs are cultured under red light, and after 8 weeks, callus is differentiated. However, callus appeared only after several weeks of cultivation under blue light, and after 15 days of inoculation of gladiolus bulbs, buds appeared first in culture under blue light, and the formed seedlings grew vigorously, while the seedlings were slender under white light. According to Ni Dexiang's research on carnation, the growth is highest under white light, followed by red, yellow, green, and blue light that inhibit growth, monochromatic light can inhibit chlorophyll synthesis, and chlorophyll synthesis needs to be in composite light conditions. Finished. With the continuous advancement of technology, white light LEDs have developed rapidly in recent years. As a new type of high-efficiency energy-saving light source, LEDs have high light efficiency, low energy consumption, safety, reliability and durability, and the wavelength is exactly in line with the spectral range formed by plant light synthesis and light form. Consistent, therefore, the application of LED plant growth lights in tissue culture is becoming more and more common.

3.Photoperiod

When in vitro culture of test tube seedlings, a certain light and dark period is used for tissue culture. The most commonly used periods are 16h light and 8h dark. Studies have shown that organs and tissues of varieties that are sensitive to short-day light tend to differentiate under short-day light, and callus production under long-day light sometimes requires dark culture, especially callus of some plants under dark culture conditions. Better in light conditions. Such as safflower, black rice tree callus. Except that some materials require dark conditions to induce callus, general culture requires certain light.

1. Prevent ultraviolet light (ultraviolet radiation reduces service life), if you need to use ultraviolet light, you must stick the original protective film on the sticky mat.

2. Cleaning the sticky mat: It is recommended to use ultrasonic wave and appropriate detergent to clean it, or clean it with water and allow to dry naturally.

3. Do not use cloth or paper towels to wipe.

4. Do not disinfect or clean with alcohol and other supplies.

1. The equipment should be moved in parallel. The maximum tilt of the box cannot exceed 45 degrees.

2. During the transportation, it is forbidden to place any bottle with liquid inside the machine; the shaker platform should be fixed for stackable shaking incubator.

3. For stackable shaker products, it is recommended to disassemble them with special lifting rods, layered packaging. ZQZY-78B / ZQZY-88B can be directly packed into boxes.

4. If the handle of the shaker is a plastic handle, it is recommended to remove it first, and remove the power cord at the same time.

5. Before packing the shaker, check whether the shaker is placed upside down. If the shaker is placed upside down, the compressor may be affected. Please put it upright, then wrap it with plastic foam, and then pack it.

6 .During the packing process, make sure that the base of the shaker is in direct contact with the bottom of the box. If there are casters on the bottom of the machine, the direct contact between the bottom casters of the machine and the bottom of the box is prohibited.

 

Method 1:
Machine model: second letter from left to right, "H" means constant temperature machine; "Q" means full temperature machine.

Method 2:
Does the machine have a compressor (black part)? No compressor means constant temperature machine.

1. The fixed fixture of our company is customized with the Shuniu brand flask as standard. If the size is found to be inappropriate during use, it is recommended to replace the Shuniu brand flask.

2. It is forbidden to spray alcohol directly to the air outlet at the rear of the machine cavity and the two ends of the ultraviolet lamp, which will directly cause damage to the cross-flow fan and the ultraviolet lamp.

3 Do not look directly at the UV lamp for a long time.

4 The equipment should be moved in parallel, and the maximum tilt of the box cannot exceed 45 degrees.

5 Installation requirements: The incubator should be placed on a solid, flat surface; strongly corrosive gas and relative humidity of about 85%; good indoor ventilation; 

the distance between the back of the oscillator and the wall should be greater than 20 cm; the right side needs to be cooled within 40CM, and there must be no obstructions;

6. When the equipment works in the "cooling" state for a long time or when it is stopped for more than three months, it should be heated as scheduled.

The tide-repellent treatment is performed every two weeks. The method is to reset the temperature to 40 ° C and open the cover to make the equipment work in the "heating" state. Each time cannot be less than 4 hours, so that the moisture in the circulation box is fully dispersed.

7. The box is equipped with UV lamp with sterilization function. Pay attention to the ultraviolet lamp radiation when using it, and it must be turned off when not in use.

8. Do not place flammable or explosive materials.
9. Keep the inside of the box clean, and often clean up debris.